As a result 36 immortalized endothelial cell lines of human mouse rat porcine and bovine origins were found for the establishment of in vitro blood brain barrier and brain endothelium models.
Immortalised human brain endothelial cell line.
However the establishment of a reliable human cell line based bbb model has proven to be difficult.
Immortalized human cerebral microvascular endothelial cell line hcmec d3.
The limited supply of primary human becs and their instability over passage number make this choice unattractive for medium high throughput studies.
The objective of this study was to generate an immortal cell line representative of specialized human brain microvascular endothelia forming the blood brain barrier bbb in vivo.
Cell differentiation was stimulated by eliminating an immortalization signal.
Our cells displayed both tight junction and efflux transporter functionalities.
Primary and immortalised human brain endothelial cells becs represent two suitable options for studying p gp function in vitro.
The hcmec d3 bbb cell line has been extensively characterized for brain endothelial phenotype and is a model of human blood brain barrier bbb function.
Blood brain barrier hcmec d3 cell line msds material safety data sheet or sds coa and coq dossiers brochures and other available documents.
A new human brain microvascular endothelial cell line has been established.
Human capillary and microvascular endothelial cells hcec were transfected with the plasmid psv3 neo coding for the sv40 large t antigen and the neomycin gene.
Here we demonstrate that the immortalized human brain endothelial cell line hcmec d3 is a useful alternative to primary brain endothelial cells as a model of the blood brain barrier for studies of central nervous system infection.
Hbmec cells express p gp claudin 1 claudin 3 occludin 55 57 zo 1 54 56 58 ß catenin icam 1 and vcam 1 some of which were also shown under our experimental conditions ve cadherin zo 1 and.
Immortalized human brain capillary endothelial cells can be used as an alternative.
Our cells will offer a promising tool for in vitro blood brain barrier models.
P glycoprotein p gp expression at the blood brain barrier prevents unwanted blood borne toxins and signalling molecules from entering the brain.
The neomycin resistant transfected cells overcame proliferative senescence and after a 6 8 wk period of crisis produced immortalization.
Our cells showed high proliferation activity while forming a monolayer.